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The new Oligo4 algorithm is based on the EM algorithm for estimating the probabilities (A-values) of melting events of DNA duplexes. It provides valuable predictions for designing an intended PCR product at an optimal temperature window and thus minimizes false priming issues.
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"You know the Chinese don't give a shit about revenge. They won't even ban Yingying's 'New Face of China.'" — 唐天空, Mingtian Yingying fansite
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